Locus Rank
7

Gene

Id
127
Gene Name
CHST12
Duplicated
True
Maternal
True
Gene Not Within Locus (Nearby
True
Gene Description
The protein encoded by this gene belongs to the sulfotransferase 2 family. It is localized to the golgi membrane, and catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin and desulfated dermatan sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage, and is distributed on the surfaces of many cells and extracellular matrices. Alternatively spliced transcript variants differing only in their 5' UTRs have been found for this gene.
Mouse Phenotype
Additional Image
Allen Brain
Allen Regions
Zfin In Situ
Zfin Brain Areas
Published Zebrafish Pehnotype
Not In Allen Brain
False
ZFIN Link
None
Allen Link
http://mouse.brain-map.org/gene/show/37594
More Additional Images
Papers
http://www.ncbi.nlm.nih.gov/pubmed/25225104 on tenascin
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121957

Locus Rank

Locus Rank
7
Genes in Region
MAD1L1 (only gene in locus), and CHST12, SNX8, FTSJ2, EIF3B, NUDT1, and ELFN1 nearby locus.
Associated Snps
GWAS Region (hg19)
chr7:1896096-2190096
Genes Skipping
SNX8 - no brain expression, seems unlikely based on pubmed; NUDT1 - also not allen brain, and nothing about neurons in any paper; FTSJ2 - cell cycle, dna repair; EIF3B - essential roles in protein synthesis
Ricopili Plot Surrounding Area
Distance On Each Side Of Locus In Above Plot
0.5 MB
Ricopili Plot
Gwas And Surrounding Region Pic
Gwas Region Pic
Genes In Locus Rank
MAD1L1
ELFN1
CHST12

Sequence 1

Ensembl Gene Name
chst12a
Harvard Allele
a252
Mutation Area - WT DNA Sequence
CCGCATTTTCATGATTGTGGGTGCCGTCTTCATGATCCTTCTTATAATCATATACTGGGATGATGTGGGGGCCTCTAATTTTTACTTGCACACTACCATCTCCGGACCCCATCCGTCACGCCTCTCTCCACAGGGCCGTCGTGGGCCTGAAAAGAAAGTAGAGGAAGACAAGGAGAGCTCGTTCTTGACGGACATAGATGCAT
Mutation Area - Mutant DNA Sequence
CCGCATTTTCATGATTGTGGGTGCCGTCTTCATGATCCTTCTTATAATCATATACTGGGATGATGTGGGGGCCTCTAATTTTTACTTGCACACTACCTCTCTCCACAGGGCCGTCGTGGGCCTGAAAAGAAAGTAGAGGAAGACAAGGAGAGCTCGTTCTTGACGGACATAGATGCAT
Guide RNA Target Sites
TGACGGATGGGGTCCGGAGATGG
Genotyping Primers
f, CCGCATTTTCATGATTGTGGGTGCCG
r, ATGCATCTATGTCCGTCAAGAACGAG
Alignment of WT and Mutant Sequences w/ gRNA Targets (cyan) and Genotyping Primers (pink) Shown on WT Sequence
Allele
25bpD
WT Genotyping Size
203
WT Protein Sequence
MALSQSKSTKMGKSRLFRIFMIVGAVFMILLIIIYWDDVGASNFYLHTTISGPHPSRLSP
QGRRGPEKKVEEDKESSFLTDIDAFVNQFLEGTADPTEQVRGEPPPGDPHNQSSEKSDEK
FVPRREWKIHLSPIDPEKKQKQENRKQLIQDVCGNKSVFDFPGKNRTFDDIPNKELDHLI
VDDRHGIIYCYVPKVACTNWKRIMIVLSESLLVDGVPYQDPLDVPQELIHNSSLHFTFNK
FWKRYGKFSRHLMKIKLKKYTKFLFVRDPFVRLISAYRNKFEQENEDFYKRFALVMLKKY
SNYTDPPASVVDAFAAGIRPSFSNFVQYLLDPSTEKEMPFNEHWRQMYRLCHPCQINYDF
VGKLETLDEDAEHLLRILRVDNIVEFPESHRNRTVSSWEQDWFANIPLETRKELYRLYEA
DFKLFGYSKPEKLLHE-
Mutant Protein Sequence
MALSQSKSTKMGKSRLFRIFMIVGAVFMILLIIIYWDDVGASNFYLHTTSLHRAVVGLKRK-
Mutant Genotyping Size
178
Ribosome Profiling Development

Sequence 2

Ensembl Gene Name
chst12b
Harvard Allele
a253
Mutation Area - WT DNA Sequence
GATGGGACTTTTTATCTGCACGCAGCTTTATCAACACCTGCGCTCCCAGCAAGTGTTGTGTTCTCTGAACGTTGGACAAGCGAGGATTTGTTTATCTGGAAAGAGTCGAGCAGGTCAGTCAAACCAAACCTGACAATCATCTCAGCTCATCCAAACAATCTAACGCACATGTTCATGCAGTCTTCCTCCAAAAACCTCAAACTTCGTCAAGCACGTCGCAAACGTATAATAAAAGAACTTTGTCAAGCCAACAGCAGTTTGATTTTCCCTGGAAAATTTAGGACATTTGACCAAATTCCAAGCAAAGAGCTGGACCATCTCATTGTGGACGATCGTCACGGTGTGATTTACTGTTTTGTACCCAAGGTAGCTTGTACCAACTGGAAACGAATCATGATTGTGCTCAGCCAGAAGCTGAAGGCACCTGATGGAGCTCCATATCTGGATCCCCTTGACATACCTTCAGCTTTATCCCATAATGCTACTGTGCATTTGACATTTAACAAGTTTTGGAGGCTTTTTGGTCATTTTTCACGTCCTCTGATGCATCACAAGCTGAAGAACTACACTAAGTTCCTCTTTGTACGGGATCCTTTTGTACGACTGATTTCTGCTTTCCGGAACAAGTTTGCTCTGCCAAATGAGGATTTCTACAAACAGTTCGGCTCCACAATGCTTCAGCGTTATGCTAATATTTCTCAGCCCCCCACTTCGGCTCAAGAGGCCTTCAGTGCAGGCATCAGACTGTCCTTTACTCACTTCATTAAGTATCTGTTAGATCCACAGACTGAGAAGGAGAAGCCGTT
Mutation Area - Mutant DNA Sequence
GATGGGACTTTTTATCTGCACGCAGCTTTATCAACACCTGCGCTCCCAGCAAGTGTTGTGTTCTCTGACGTTGGACAAGCGAGGATTTGTTTATCTGGAAAGAGTCGAGCAGGTCAGTCAAACCAAACCGGACAATCATCTCAGCTCATCAAAACAATCTAACGCACATGTTCATGCAGTCTTCCTCCAAAAACCTCAAACTTCGTCAAGCGCGTCGCAAACGTATAATAAAAGAACTTTGTTTAGCCAACAGCAGTTTGAACTTCCCTGGAAAATTTAGGACATTTGACCAAATTCCAAGCAAAGAGCTGGACCAAGAGGCCTTCAGTGCAGGTATCAGACTGTCTTTTACTCACTTCATTAAGTATCTGTTAGATCCACAGACTGAGAAGGAGAAGCCGTT
Guide RNA Target Sites
GTGTTGTGTTCTCTGAACGTTGG
GACGTGCTTGACGAAGTTTGAGG
AGAGCTGGACCATCTCATTGTGG
GAAGGCCTCTTGAGCCGAAGTGG
Genotyping Primers
f, GATGGGACTTTTTATCTGCACGCAGC
r, AACGGCTTCTCCTTCTCAGTCTGTGG
Alignment of WT and Mutant Sequences w/ gRNA Targets (cyan) and Genotyping Primers (pink) Shown on WT Sequence
Allele
1bpD,402bpD
WT Genotyping Size
806
WT Protein Sequence
MVRQLQFFLLLGTLFIIFFIIIHWDDVKDGTFYLHAALSTPALPASVVFSERWTSEDLFI
WKESSRSVKPNLTIISAHPNNLTHMFMQSSSKNLKLRQARRKRIIKELCQANSSLIFPGK
FRTFDQIPSKELDHLIVDDRHGVIYCFVPKVACTNWKRIMIVLSQKLKAPDGAPYLDPLD
IPSALSHNATVHLTFNKFWRLFGHFSRPLMHHKLKNYTKFLFVRDPFVRLISAFRNKFAL
PNEDFYKQFGSTMLQRYANISQPPTSAQEAFSAGIRLSFTHFIKYLLDPQTEKEKPFNEH
WQQMHRLCHPCQIDYDFVGKLETLHEDTEHLLKILGLSNQIRFPPGYRNRTAVKWEREWF
ANVSVADRRKLYSLYEADFRLFGYNKPETLF-
Mutant Protein Sequence
MVRQLQFFLLLGTLFIIFFIIIHWDDVKDGTFYLHAALSTPALPASVVFSERWTSEDLFIWKESSRS
VKPNRTIISAHQNNLTHMFMQSSSKNLKLRQARRKRIIKELCLANSSLNFPGKFRTFDQIPSKELDQE
AFSAGIRLSFTHFIKYLLDPQTEKEKPFNEHWQQMHRLCHPCQIDYDFVGKLETLHEDTEHLLKILGL
SNQIRFPPGYRNRTAVKWEREWFANVSVADRRKLYSLYEADFRLFGYNKPETLF-
Mutant Genotyping Size
403

Behavior Data

Behavior Data Summary
None
Behavior Data Description 1
Dark flash block 1 start Merged section Pvalue = not significant 14 hethet vs 8 homhom ribgraph_mean_ribbon_fullboutdata_dpix_a0darkflash103.png
Behavior Data Graph 1
Behavior Data Description 2
Day all prepulse tap Merged section Pvalue = 0.0132 14 hethet vs 8 homhom ribgraph_mean_ribbon_fullboutdata_dpix_dayprepulseinhibition100d.png
Behavior Data Graph 2
Behavior Data Description 3
Features of movement entire protocol Graph Pvalue = not significant Merged section Pvalue = not significant 14 hethet vs 8 homhom ribgraph_mean_ribbonbout_aveboutdisp_10min_combo.png
Behavior Data Graph 3
Behavior Data Description 4
Frequency of movement entire protocol Graph Pvalue = not significant Merged section Pvalue = not significant 14 hethet vs 8 homhom ribgraph_mean_ribbonbout_dpixnumberofbouts_10min_combo.png
Behavior Data Graph 4
Behavior Data Description 5
Location in well entire protocol Graph Pvalue = 0.0478 Merged section Pvalue = not significant 14 hethet vs 8 homhom ribgraph_mean_ribbonbout_averhofrac_10min_combo.png
Behavior Data Graph 5
Behavior Data Description 6
Frequency of movement Graph Pvalue = 0.014 18 hethet vs 15 hethom ribgraph_mean_ribbon_distminper_10min_day2nightstim.png
Behavior Data Graph 6
Behavior Data Description 7
Strong tap Graph Pvalue = 0.00134 14 hethet vs 8 homhom boxgraph_pd__ribgraph_mean_ribbon_speedresponse_dist_nightprepulseinhibition102.png
Behavior Data Graph 7
Behavior Data Description 8
Tap habituation Graph Pvalue = 0.00459 18 hethet vs 9 homhom boxgraph_pd__ribgraph_mean_ribbon_displacement_dist_cdaytaphab1.png
Behavior Data Graph 8
Behavior Data Description 9
None
Behavior Data Graph 9
None
Behavior Data Description 10
None
Behavior Data Graph 10
None
Behavior Data Description 11
None
Behavior Data Graph 11
None
Behavior Data Description 12
None
Behavior Data Graph 12
None
Behavior Data Description 13
None
Behavior Data Graph 13
None
Behavior Data Description 14
None
Behavior Data Graph 14
None