Locus Rank
20

Gene

Id
257
Gene Name
R3HDM2
Duplicated
False
Maternal
False
Gene Not Within Locus (Nearby
False
Gene Description
Mouse Phenotype
Additional Image
Allen Brain
Allen Regions
Zfin In Situ
Zfin Brain Areas
Published Zebrafish Pehnotype
Not In Allen Brain
False
ZFIN Link
None
Allen Link
http://mouse.brain-map.org/gene/show/47591
More Additional Images
Papers

Locus Rank

Locus Rank
20
Genes in Region
LRP1 MYO1A NAB2 NDUFA4L2 NXPH4 R3HDM2 SHMT2 STAC3 STAT6 TAC3 TMEM194A
Associated Snps
rs12826178 (21)
rs324017 (103)
GWAS Region (hg19)
chr12:57428314-57682971
Genes Skipping
MYO1A - no allen brain, myosin; NDUFA4L2 - no allen brain, nadh dehydrogenase; NXPH4 - high in hindbrain of allen and jax knockout doesn't sound promising . . .; STAC3 - muscle (there is one 2015 paper hinting at calcium channels in brain, but zfin shows muscle expression only); TAC3 - is neuro, but a lot is known and seems like it's not this gene (has to do with puberty though . . ); TMEM194A - nothing is known, but not in allen brain
Ricopili Plot Surrounding Area
Distance On Each Side Of Locus In Above Plot
0.2 MB
Ricopili Plot
Gwas And Surrounding Region Pic
Gwas Region Pic
Genes In Locus Rank
NXPH4
R3HDM2
NAB2
LRP1
SHMT2
STAT6

Sequence 1

Ensembl Gene Name
R3HDM2
Harvard Allele
a225
Mutation Area - WT DNA Sequence
ggcctgtgcacaataagctcctcatggtgtgtgtttcagCCCCGAGCAGCGCTTCTCTGAGCACATTAAAGACGAGCGCAACATGGACTTCCAGAAGAAGTTCATCCTGAAGAGAGACGACGCCAGCATGGACAAGGATGATAACCAGgtgtgtgttgcagagttttcgctcctactagcactgagcgatttcccctaaagtcaaattaggatgagtgatctactgtccatgattatttattgaatatcaacgttgaagaactgatattaaagcacacattcccaatcgagctaaagtgttcaattcatcgaggttttcatttaggtcgtatcacccagaactgcttaatcaccaaatgtgttgtttatttatttaagatgaaaatgatcttttttatctgtgttgttcagATCCGCGTGCCGCTGCAGGACGGCCGTCGCAGTAAATCCATCGAGGAGCGAGAGGAAGAGTACCAGCGCGTCAGAGATCGCATTTTTGCACGAGAAgtaagaaaataatccaatgcttcaggaacagcgttactt
Mutation Area - Mutant DNA Sequence
ggcctgtgcacaataagctcctcatggtgtgtgtttcagCCCTGAGCAGCGCTTCTCTGAGCACATTAAAGACGAGCGCAACATGGACTTCCAGAAGAAGTTCATCCTGAAGAGAGACGACGCAAGTTGTCCGGTGCCACTTTTTCAAGTTGATAACGGACAAGGATGATAACCAGGTGTGTGTTGCAGAGTTTCCGCTCCTACTAGCACTGAGCGATTTTCCCTAAAGTCAAATTAGGATGAGTGATCTACTGTCCATGATTATTTATTGAATATCAACGTTGAAGAACTGATATTAAAGCACACATTCCCAATCGAGCTAAAGTGTTCAATTCATCGAGGTTTTCATTTAGGTCGTATCACCCAGAACTGCTTAATCACCAAATGTGTTGTTTATTTATTTAAGATGAAAATGATCTTTTTTATCTGTGTTGTTCAGATCCGTGTGCCGCTGCAGGACGGCCGTCGCAGTAAATCCATCGAGGAGCGAGAGGAAGAGTACCAGCGCGTCAGAGATCGCATTTTTGCACGAGAAgtaagaaaatactccaatgcttcaggaacagcgttactt
Guide RNA Target Sites
GCTCAGAGAAGCGCTGCTCGGGG
GAAGAGAGACGACGCCAGCATGG
CTGCGACGGCCGTCCTGCAGCGG
AAATGCGATCTCTGACGCGCTGG
Genotyping Primers
f, ggcctgtgcacaataagctcctc
r, aagtaacgctgttcctgaagcattgg
Alignment of WT and Mutant Sequences w/ gRNA Targets (cyan) and Genotyping Primers (pink) Shown on WT Sequence
Allele
28bpI
WT Genotyping Size
546
WT Protein Sequence
QSNAKLKLVRSLAVCEESSGPFSTDGPPESQDIIQLHISCPSDKEEEKSSKDESENEERE
KKDKTPRKMLSRDSSQEYTDSTGIDVHEFLVNTLKNNPRDRMMLLKLEQDILEFISDENN
QYKKFPQMTSYHRMLLHRVAAYFGMDHNVDQTGKAVIIVQIDSLLVIPEQRFSEHIKDER
NMDFQKKFILKRDDASMDKDDNQIRVPLQDGRRSKSIEEREEEYQRVRDRIFARESSQNG
YINDNRGNRESSSRASSSRQSSTDSDMKSLEPRPWSSTDSDSSNRTLRPPVTKASSFSGI
SILTRGDSLGSKGSQGSARGSRTGLPTITPDLCPPSQQGRGLLPCPQNCPSGAPQAPNQP
PAPPLLPTPTHTHAISAHNHMMPQGDELTQGFGQMSLSRQGSSEAPEPPSLYQPPPTLLT
QQLTQHPPPQPSYIMPPPPSGYQPAAPHTHTHPPPPPPPPAQPILQTAPPAQGYMQAPPP
PIQVQYYSTAGQFPPSGQQFRSISHQVSFPTQRSQPLPPPAPPSAPLQPMMTNQQPAYQG
MMGVQQPQSSSMMNTQRTAINGQMTGMMVQYPPMPSYHYPVANEGQPVVQQQYQQPVMVP
ASQSVQGAMPTAAPMPVYYGVLTPTQQNSTSNPLTVGYLQPPSSEQYQLTQSASPCSPQP
IQQQYSGVPPPGPGVMVMQLSVPNGPQPTQNPPLVQWNPCKYYSMEQRGSKPEPLYNTHT
HTHTHTHVVSSPLASPTQSPAPSPSGSVNSVCPGLGPLPLISQFPRPGGPAQADGRYSLL
GQPLQYSLCPPPIMHNHNQSSYSSHQNQGVMKHGARGKRQTLKSASTDLGTTDMVVSRVL
EVTDLPEGISRPEAEKLFNQLSLCGAKIQWLKDPQCPRGPGGGRGDGSDPAHLYTVVAVF
PNTMAAQNASFKLNNSGGGLFKLRAAKKNYDLRVLERASSQ
Mutant Protein Sequence
QSNAKLKLVRSLAVCEESSGPFSTDGPPESQDIIQLHISCPSDKEEEKSSKDESENEERE
KKDKTPRKMLSRDSSQEYTDSTGIDVHEFLVNTLKNNPRDRMMLLKLEQDILEFISDENN
QYKKFPQMTSYHRMLLHRVAAYFGMDHNVDQTGKAVIIVQIDSLLVIPEQRFSEHIKDERN
MDFQKKFILKRDDASCPVPLFQVDNGQG-
Mutant Genotyping Size
574

Behavior Data

Behavior Data Summary
None
Behavior Data Description 1
Dark flash block 1 start Merged section Pvalue = 0.0243 24 het vs 42 hom ribgraph_mean_ribbon_fullboutdata_dpix_a0darkflash103.png
Behavior Data Graph 1
Behavior Data Description 2
Day all prepulse tap Merged section Pvalue = not significant 24 het vs 42 hom ribgraph_mean_ribbon_fullboutdata_dpix_dayprepulseinhibition100d.png
Behavior Data Graph 2
Behavior Data Description 3
Features of movement entire protocol Graph Pvalue = not significant Merged section Pvalue = not significant 24 het vs 42 hom ribgraph_mean_ribbonbout_aveboutdisp_10min_combo.png
Behavior Data Graph 3
Behavior Data Description 4
Frequency of movement entire protocol Graph Pvalue = not significant Merged section Pvalue = not significant 24 het vs 42 hom ribgraph_mean_ribbonbout_dpixnumberofbouts_10min_combo.png
Behavior Data Graph 4
Behavior Data Description 5
Location in well entire protocol Graph Pvalue = not significant Merged section Pvalue = not significant 24 het vs 42 hom ribgraph_mean_ribbonbout_averhofrac_10min_combo.png
Behavior Data Graph 5
Behavior Data Description 6
None
Behavior Data Graph 6
None
Behavior Data Description 7
None
Behavior Data Graph 7
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Behavior Data Description 8
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Behavior Data Graph 8
None
Behavior Data Description 9
None
Behavior Data Graph 9
None
Behavior Data Description 10
None
Behavior Data Graph 10
None
Behavior Data Description 11
None
Behavior Data Graph 11
None
Behavior Data Description 12
None
Behavior Data Graph 12
None
Behavior Data Description 13
None
Behavior Data Graph 13
None
Behavior Data Description 14
None
Behavior Data Graph 14
None